I told her about the time I cut open a zebrafish that hadn't actually died despite leaving it in the anesthetizing fluid for the proper amount of time, and the fact its gills had long been still. It began bleeding all over the sylgard and twitching, and my supervisor told me I couldn't put it back in the fluid and risk contaminating it. I wanted to put the fish out of its misery as quickly as possible while preserving the body for dissection, so I clipped its spinal cord and rinsed it off with NZR solution.